GST Antikörper (DyLight 549)

Produktnummer ABIN6720731

Produktdetails anti-GST Antikörper (DyLight 549)

Target: GST (Glutathione S Transferase (GST))

Reaktivität: Tag

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GST Antikörper ist konjugiert mit DyLight 549

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].  Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.

Immunogen:

Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.

Immunogen Type: Native Protein

Isotyp: IgG

Labeling Ratio: 2.6

Anwendungsinformationen

Applikationshinweise:

Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: 1:10,000 - 1:25,000

IF Microscopy Dilution: >1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Details zu GST

Target: GST (Glutathione S Transferase (GST))

Andere Bezeichnung: GST (GST Produkte)

Synonyme: GST antikoerper, GST 13-13 antikoerper, GST13 antikoerper, GST13-13 antikoerper, GSTK1-1 antikoerper, hGSTK1 antikoerper, 1500002K10Rik antikoerper, Gst antikoerper, Ptgds2 antikoerper, glutathione S-transferase kappa 1 antikoerper, microsomal glutathione S-transferase 1 antikoerper, hematopoietic prostaglandin D synthase antikoerper, GSTK1 antikoerper, Mgst1 antikoerper, Hpgds antikoerper

Hintergrund:

Synonyms: rabbit anti-GST antibody DyLight™549 conjugation, DyLight™549 conjugated rabbit anti-GST antibody, Glutathione-S-Transferase

Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.