GST Antikörper (DyLight 549)

Produktnummer ABIN6720729

Hersteller Produkt- Nr.: 200-342-200

Dieses Anti-GST-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von GST in WB, ELISA, FLISA und FM. Geeignet für Schistosoma japonicum.

Kurzübersicht für GST Antikörper (DyLight 549) (ABIN6720729)

Target: GST (Glutathione S Transferase (GST))

Reaktivität: Schistosoma japonicum

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GST Antikörper ist konjugiert mit DyLight 549

Applikation: Western Blotting (WB), ELISA, FLISA, Fluorescence Microscopy (FM)

Klon: 3D4

Produktdetails anti-GST Antikörper (DyLight 549)

Hersteller Produkt- Nr.: 200-342-200

Hersteller: Rockland

Verwendungszweck: GST Antibody Dylight™ 549 Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and purified and partially purified Glutathione-S-Transferase (GST).

Aufreinigung: This product was prepared from tissue culture supernatant by Protein A affinity chromatography.

Immunogen: BALB/c mice were immunized with GST from full length Schistosoma japonicum protein.  A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology.

Isotyp: IgG1 kappa

Labeling Ratio: 2.9

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Kommentare:

Anti-GST DyLight™ 549 has ben tested by ELISA and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu GST

Target: GST (Glutathione S Transferase (GST))

Andere Bezeichnung: GST

Hintergrund:

Synonyms: mouse anti-GST antibody DyLight™ 549 conjugation, DyLight™549 conjugated mouse anti-GST antibody, Glutathione-S-Transferase, Anti-GST monoclonal antibody

Background: Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.