GFP Antikörper (DyLight 680)

Produktnummer ABIN6720707

Hersteller Produkt- Nr.: 600-144-215

Der Ziege Polyklonal Anti-GFP-Antikörper wurde für WB, ELISA, FM und FLISA validiert. Er ist geeignet, GFP in Proben von Aequorea victoria zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für GFP Antikörper (DyLight 680) (ABIN6720707)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist konjugiert mit DyLight 680

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), FLISA

Produktdetails anti-GFP Antikörper (DyLight 680)

Hersteller Produkt- Nr.: 600-144-215

Hersteller: Rockland

Verwendungszweck: GFP Antibody Dylight™ 680 Conjugated

Spezifität: Wt, rGFP, eGFP

Kreuzreaktivität (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Isotyp: IgG

Labeling Ratio: 2.8

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

ELISA_Dilution: 1:20,000 - 1:40,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Kommentare:

Suggested Applications: IF
The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-GFP Antikörper (DyLight 680) (ABIN6720707)

Ferrari, Malardé, Grassart, Salavessa, Nigro, Descorps-Declere, Rohde, Schnupf, Masson, Arras, Loew, Sansonetti, Sauvonnet: "Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, Issue 27, pp. 13582-13591, (2020) (PubMed).

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Hintergrund:

Synonyms: goat anti-GFP Antibody DyLight™ 680 Conjugation, DyLight™ 680 conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms such as the LICOR Odyssey Imaging system. DyLight conjugated antibodies are effective alternatives to Alexa 680 and IR Dye 680 conjugates.

UniProt: P42212