GFP Antikörper (DyLight 488)

Produktnummer ABIN6720701

Hersteller Produkt- Nr.: 600-141-215

Der Ziege Polyklonal Anti-GFP-Antikörper wurde für WB, ELISA, FM, DB und FLISA validiert. Er ist geeignet, GFP in Proben von Aequorea victoria zu detektieren. Es sind 22+ Publikationen verfügbar.

Kurzübersicht für GFP Antikörper (DyLight 488) (ABIN6720701)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist konjugiert mit DyLight 488

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Dot Blot (DB), FLISA

Produktdetails anti-GFP Antikörper (DyLight 488)

Hersteller Produkt- Nr.: 600-141-215

Hersteller: Rockland

Verwendungszweck: GFP Antibody Dylight™ 488 Conjugated

Spezifität: Wt, rGFP, eGFP

Kreuzreaktivität (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Aufreinigung: GFP Dylight™ 488 Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Isotyp: IgG

Labeling Ratio: 5.7

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:10,000

ELISA_Dilution: 1:20,000 - 1:40,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000

Other: User Optimized

Kommentare:

Suggested Applications: FC, IF, IHC, IP, Multiplex
Anti-GFP Dylight™ 488 Conjugated Antibody has been tested by dot blot and western blot. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Conjugated anti-GFP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-GFP Antikörper (DyLight 488) (ABIN6720701)

Park, Kim, Alabdalla, Mishra, Mchaourab: "Generation and characterization of a zebrafish knockout model of abcb4, a homolog of the human multidrug efflux transporter P-glycoprotein." in: Human genomics, Vol. 17, Issue 1, pp. 84, (2023) (PubMed).

Annunziata, Rasa, Krepelova, Lu, Minetti, Omrani, Nunna, Adam, Käppel, Neri: "Paneth cells drive intestinal stem cell competition and clonality in aging and calorie restriction." in: European journal of cell biology, Vol. 101, Issue 4, pp. 151282, (2022) (PubMed).

Kim, Leng, Park, Sorets, Kim, Shostak, Embalabala, Mlouk, Katdare, Rose, Sturgeon, Neal, Ao, Wang, Sofroniew, Brunger, McMahon, Schrag, Kampmann, Lippmann: "Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin." in: Nature communications, Vol. 13, Issue 1, pp. 6581, (2022) (PubMed).

Paterson, Lämmermann: "Macrophage network dynamics depend on haptokinesis for optimal local surveillance." in: eLife, Vol. 11, (2022) (PubMed).

Kim, Jiang: "Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin." in: STAR protocols, Vol. 2, Issue 2, pp. 100443, (2022) (PubMed).

Bhargava, Williart, Maurin, Davidson, Jouve, Piel, Lahaye, Manel: "Inhibition of HIV infection by structural proteins of the inner nuclear membrane is associated with reduced chromatin dynamics." in: Cell reports, Vol. 36, Issue 13, pp. 109763, (2022) (PubMed).

Lee, Zhao, Wong, Chrysostomou, Crowston, Bui: "The Effect of Aging on Retinal Function and Retinal Ganglion Cell Morphology Following Intraocular Pressure Elevation." in: Frontiers in aging neuroscience, Vol. 14, pp. 859265, (2022) (PubMed).

Wang, Wong, Lee, Bui, Dudczig, Vessey, Fletcher: "Retinal ganglion cell dysfunction in mice following acute intraocular pressure is exacerbated by P2X7 receptor knockout." in: Scientific reports, Vol. 11, Issue 1, pp. 4184, (2021) (PubMed).

Freter, Falletta, Omrani, Rasa, Herbert, Annunziata, Minetti, Krepelova, Adam, Käppel, Rüdiger, Wang, Goding, Neri: "Establishment of a fluorescent reporter of RNA-polymerase II activity to identify dormant cells." in: Nature communications, Vol. 12, Issue 1, pp. 3318, (2021) (PubMed).

Xu, Brawner, Li, Liu, Kim, Xue, Pang, Kim, Hart, Liu, Jiang: "OLIG2 Drives Abnormal Neurodevelopmental Phenotypes in Human iPSC-Based Organoid and Chimeric Mouse Models of Down Syndrome." in: Cell stem cell, Vol. 24, Issue 6, pp. 908-926.e8, (2020) (PubMed).

Russo, Latour, McGavern: "Distinct myeloid cell subsets promote meningeal remodeling and vascular repair after mild traumatic brain injury." in: Nature immunology, Vol. 19, Issue 5, pp. 442-452, (2019) (PubMed).

Fletcher, Greferath, Saha, Anderson, Vessey: "Ganglion Cell Assessment in Rodents with Retinal Degeneration." in: Methods in molecular biology (Clifton, N.J.), Vol. 1753, pp. 261-273, (2019) (PubMed).

Gentili, Lahaye, Nadalin, Nader, Puig Lombardi, Herve, De Silva, Rookhuizen, Zueva, Goudot, Maurin, Bochnakian, Amigorena, Piel, Fachinetti, Londoño-Vallejo, Manel: "The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus." in: Cell reports, Vol. 26, Issue 9, pp. 2377-2393.e13, (2019) (PubMed).

Kolter, Feuerstein, Zeis, Hagemeyer, Paterson, dErrico, Baasch, Amann, Masuda, Lösslein, Gharun, Meyer-Luehmann, Waskow, Franzke, Grün, Lämmermann, Prinz, Henneke: "A Subset of Skin Macrophages Contributes to the Surveillance and Regeneration of Local Nerves." in: Immunity, Vol. 50, Issue 6, pp. 1482-1497.e7, (2019) (PubMed).

Haas, Meiler, Geiger, Vogt, Preuss, Kochs, Pichlmair: "Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity." in: PLoS pathogens, Vol. 14, Issue 4, pp. e1006980, (2018) (PubMed).

Begolly, Olschowka, Love, Williams, OBanion: "Fractionation enhances acute oligodendrocyte progenitor cell radiation sensitivity and leads to long term depletion." in: Glia, Vol. 66, Issue 4, pp. 846-861, (2018) (PubMed).

Bongianino, Denegri, Mazzanti, Lodola, Vollero, Boncompagni, Fasciano, Rizzo, Mangione, Barbaro, Di Fonso, Napolitano, Auricchio, Protasi, Priori: "Allele-Specific Silencing of Mutant mRNA Rescues Ultrastructural and Arrhythmic Phenotype in Mice Carriers of the R4496C Mutation in the Ryanodine Receptor Gene (RYR2)." in: Circulation research, Vol. 121, Issue 5, pp. 525-536, (2017) (PubMed).

Dufour, Gillet, Frankel, Weibel, Emonet: "Direct Correlation between Motile Behavior and Protein Abundance in Single Cells." in: PLoS computational biology, Vol. 12, Issue 9, pp. e1005041, (2017) (PubMed).

"Correction: Direct Correlation between Motile Behavior and Protein Abundance in Single Cells." in: PLoS computational biology, Vol. 12, Issue 9, pp. e1005149, (2016) (PubMed).

Ho, Vessey, Fletcher: "Immunolocalization of the P2X4 receptor on neurons and glia in the mammalian retina." in: Neuroscience, Vol. 277, pp. 55-71, (2015) (PubMed).

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Hintergrund:

Synonyms: goat anti-GFP Antibody DyLight™ 488 Conjugation, DyLight™ 488 conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Background: GFP Dylight™ 488 Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

UniProt: P42212