GFP Antikörper (DyLight 488)

Produktnummer ABIN6720700

Hersteller Produkt- Nr.: 200-341-215

Produktdetails anti-GFP Antikörper (DyLight 488)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFP Antikörper ist konjugiert mit DyLight 488

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), FLISA

Hersteller Produkt- Nr.: 200-341-215

Hersteller: Rockland

Verwendungszweck: GFP Antibody Dylight™ 488 Conjugated

Spezifität: Wt, rGFP, eGFP

Kreuzreaktivität (Details): Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum.

Aufreinigung: GFP Dylight™ 488 Conjugated Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography.

Immunogen: BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.  A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology.

Klon: 9F9-F9

Isotyp: IgG1 kappa

Labeling Ratio: 3.2

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Kommentare:

Suggested Applications: IF
Anti-GFP Dylight™ 488 Conjugated has been tested by ELISA and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-GFP Antikörper (DyLight 488) (ABIN6720700)

Khosravi, Schindele, Gladilin, Dunemann, Rutten, Puchta, Houben: "Application of Aptamers Improves CRISPR-Based Live Imaging of Plant Telomeres." in: Frontiers in plant science, Vol. 11, pp. 1254, (2020) (PubMed).

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP (GFP Produkte)

Hintergrund:

Synonyms: mouse anti-GFP Antibody Dylight™ 488 Conjugation, Dylight™ 488 Conjugated mouse anti-GFP Antibody, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Background: GFP monoclonal antibody is Dylight™ 488 Conjugated. Labeled GFP Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods.

UniProt: P42212