DYKDDDDK Tag Antikörper (DyLight 800)

Produktnummer ABIN6720690

Hersteller Produkt- Nr.: 200-345-383

Zitiert in 1 Publikation. Der DyLight 800-konjugierte Maus Monoklonal anti-DYKDDDDK Tag Antikörper (Klon 29E4-G7) (ABIN6720690) detektiert spezifisch DYKDDDDK Tag in WB, ELISA, FACS, DB, FM und FLISA.

Kurzübersicht für DYKDDDDK Tag Antikörper (DyLight 800) (ABIN6720690)

Target: DYKDDDDK Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DYKDDDDK Tag Antikörper ist konjugiert mit DyLight 800

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Dot Blot (DB), Fluorescence Microscopy (FM), FLISA

Klon: 29E4-G7

Produktdetails anti-DYKDDDDK Tag Antikörper (DyLight 800)

Hersteller Produkt- Nr.: 200-345-383

Hersteller: Rockland

Verwendungszweck: DYKDDDDK Tag (Anti-FLAG®) Antibody Dylight™ 800 Conjugated

Kreuzreaktivität (Details): The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Aufreinigung: This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays.

Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.

Isotyp: IgG2a kappa

Labeling Ratio: 1.6

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

ELISA_Dilution: 1:10,000 - 1:25,000

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Kommentare:

Anti-FLAG DyLight™800 has been tested by ELISA, dot blot, and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-DYKDDDDK Tag Antikörper (DyLight 800) (ABIN6720690)

Warmack, Pang, Peluso, Lowenson, Ong, Torres, Clarke: "Human Protein-l-isoaspartate O-Methyltransferase Domain-Containing Protein 1 (PCMTD1) Associates with Cullin-RING Ligase Proteins." in: Biochemistry, Vol. 61, Issue 10, pp. 879-894, (2022) (PubMed).

Details zu DYKDDDDK Tag

Target: DYKDDDDK Tag

Hintergrund:

Synonyms: mouse anti-FLAG™ tag DyLight™800 conjugation, Enterokinase Cleavage Site (ECS), DyLight™800 conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

Background: Antibody for the detection of FLAG™ recognizes FLAG™ and is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. Antibody for the detection of FLAG™ can be used to identify fusion proteins containing the FLAG™ epitope. Antibody for the detection of FLAG™ recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.