Caspase 2 Antikörper (AA 170-325)

Produktnummer ABIN6719550

Dieses Anti-Caspase 2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Caspase 2 in WB, ELISA und IHC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für Caspase 2 Antikörper (AA 170-325) (ABIN6719550)

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-Caspase 2 Antikörper

Bindungsspezifität: AA 170-325

Verwendungszweck: Anti-Caspase-2/CASP2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Caspase-2/CASP2 Antibody Picoband® (ABIN6719550). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human Caspase-2 recombinant protein (Position: G170-T325).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Bergeron, L., Perez, G. I., Macdonald, G., Shi, L., Sun, Y., Jurisicova, A., Varmuza, S., Latham, K. E., Flaws, J. A., Salter, J. C. M., Hara, H., Moskowitz, M. A., Li, E., Greenberg, A., Tilly, J. L., Yuan, J. : Defects in regulation of apoptosis in caspase-2-deficient mice. Genes Dev. 12: 1304-1314, 1998. 2. Tinel, A., Tschopp, J. : The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress. Science 304: 843-846, 2004.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Caspase 2

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Andere Bezeichnung: CASP2

Hintergrund:

Synonyms: Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, NEDD-2, Protease ICH-1, Caspase-2 subunit p18, Caspase-2 subunit p13, Caspase-2 subunit p12, CASP2, ICH1, NEDD2

Tissue Specificity: Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.

Background: Caspase-2, which is involved in stress-induced apoptosis, is recruited into a large protein complex, the molecular composition of which remains elusive. activation of caspase-2 occurs in a complex that contains the death domain-containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD. Increased PIDD expression resulted in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli. Caspase-2 acts both as a positive and negative cell death effector, depending upon cell lineage and stage of development.

Molekulargewicht: 51 kDa

Gen-ID: 835

UniProt: P42575

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Neurotrophin Signalübertragung