Galectin 3 Antikörper (AA 139-250)

Produktnummer ABIN6719486

Der Maus Monoklonal Anti-Galectin 3 Antikörper wurde für WB, IHC, ICC, IF und FACS validiert. Er ist geeignet, Galectin 3 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Galectin 3 Antikörper (AA 139-250) (ABIN6719486)

Target: Galectin 3 (LGALS3)

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Galectin 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 12B12

Produktdetails anti-Galectin 3 Antikörper

Bindungsspezifität: AA 139-250

Verwendungszweck: Anti-Galectin 3/LGALS3 Antibody (monoclonal, 12B12)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Galectin 3/LGALS3 Antibody (monoclonal, 12B12) (ABIN6719486). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequence identity with mouse and rat Galectin 3, respectively.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Galectin 3

Target: Galectin 3 (LGALS3)

Andere Bezeichnung: LGALS3

Hintergrund:

Synonyms: Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, LGALS3, MAC2

Tissue Specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.

Background: SAM domain and HD domain-containing protein 1 is a protein that in humans is encoded by the SAMHD1 gene. This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.

Molekulargewicht: 28 kDa

Gen-ID: 3958

UniProt: P17931

Pathways: RTK Signalweg