PARN Antikörper (AA 1-301)

Produktnummer ABIN6719422

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PARN in WB, IHC, IF, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Affe.

Kurzübersicht für PARN Antikörper (AA 1-301) (ABIN6719422)

Target: PARN (Poly A Specific Ribonuclease (PARN))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PARN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 11D8

Produktdetails anti-PARN Antikörper

Bindungsspezifität: AA 1-301

Verwendungszweck: Anti-PARN Antibody Picoband® (monoclonal, 11D8)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PARN Antibody Picoband® (monoclonal, 11D8) (ABIN6719422). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human PARN recombinant protein (Position: M1-Y301).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1."Entrez Gene: PARN poly(A)-specific ribonuclease (deadenylation nuclease)". Buiting K, Korner C, Ulrich B, Wahle E, Horsthemke B (May 2000). "The human gene for the poly(A)-specific ribonuclease (PARN) maps to 16p13 and has a truncated copy in the Prader-Willi/Angelman syndrome region on 15q11→q13". Cytogenet Cell Genet. 87 (1-2): 125-31.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu PARN

Target: PARN (Poly A Specific Ribonuclease (PARN))

Andere Bezeichnung: PARN

Hintergrund:

Synonyms: Poly (A)-specific ribonuclease PARN, Deadenylating nuclease, Deadenylation nuclease, Polyadenylate-specific ribonuclease, PARN, DAN

Tissue Specificity: Ubiquitous.

Background: Poly (A)-specific ribonuclease (PARN), also known as polyadenylate-specific ribonuclease or deadenylating nuclease (DAN), is an enzyme that in humans is encoded by the PARN gene. The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly (A) as the substrate, hence, efficiently degrades poly (A) tails of mRNAs. Exonucleolytic degradation of the poly (A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons.

Molekulargewicht: 78 kDa

Gen-ID: 5073

UniProt: O95453