BID Antikörper (AA 1-195) (DyLight 550)

Produktnummer ABIN7354548

Dieses Anti-BID-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von BID in FACS. Geeignet für Human.

Kurzübersicht für BID Antikörper (AA 1-195) (DyLight 550) (ABIN7354548)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BID Antikörper ist konjugiert mit DyLight 550

Applikation: Flow Cytometry (FACS)

Produktdetails anti-BID Antikörper (DyLight 550)

Bindungsspezifität: AA 1-195

Verwendungszweck: Anti-Human Bid DyLight® 550 conjugated Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Human Bid DyLight® 550 conjugated Antibody (ABIN5518895)-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequence identity with mouse and rat Bid, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Chou, J. J., Li, H., Salvesen, G. S., Yuan, J., Wagner, G. Solution structure of BID, an intracellular amplifier of apoptotic signaling. Cell 95: 615-624, 1999. 2. Luo, X., Budihardjo, I., Zou, H., Slaughter, C., Wang, X. Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell 94: 481-490, 1998. 3. Wang, K., Yin, X.-M., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Keck, C. L., Zimonjic, D. B., Popescu, N. C., Korsmeyer, S. J. BID, a proapoptotic BCL-2 family member, is localized to mouse chromosome 6 and human chromosome 22q11. Genomics 53: 235-238, 1998.

Kommentare:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Details zu BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Andere Bezeichnung: BID

Hintergrund:

Synonyms: BH3-interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist p15, p15 BID, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID, BID

Tissue Specificity: Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).

Background: BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.

Molekulargewicht: 39 kDa

Gen-ID: 637

UniProt: P55957

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity