Cyclin D1 Antikörper (AA 61-110)

Produktnummer ABIN671016

Dieses Anti-Cyclin D1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Cyclin D1 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) und IHC (fro). Geeignet für Human, Maus, Ratte, Rind (Kuh), Kaninchen und Schaf. Dieses Primary Antibody wurde in 15+ Publikationen zitiert.

Kurzübersicht für Cyclin D1 Antikörper (AA 61-110) (ABIN671016)

Target: Cyclin D1 (CCND1)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin D1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Cyclin D1 Antikörper

Bindungsspezifität: AA 61-110

Kreuzreaktivität: Rind (Kuh), Human, Maus, Kaninchen, Ratte, Schaf

Homologie: Dog

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Cyclin D1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-Cyclin D1 Antikörper (ABIN671016)

Jiang, Xie, Cai, Ren, Ma: "Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/β-catenin pathway." in: BMC microbiology, Vol. 17, Issue 1, pp. 124, (2017) (PubMed).

Wang, Cao, Sun, Dai, Fang, Li, Yan, Mao, Zhang: "Total flavonoid aglycones extract in Radix scutellariae inhibits lung carcinoma and lung metastasis by affecting cell cycle and DNA synthesis." in: Journal of ethnopharmacology, Vol. 194, pp. 269-279, (2016) (PubMed).

Zhang, Pan, Xu, Niu, Ma, Xu: "Interleukin 18 augments growth ability via NF-?B and p38/ATF2 pathways by targeting cyclin B1, cyclin B2, cyclin A2, and Bcl-2 in BRL-3A rat liver cells." in: Gene, Vol. 563, Issue 1, pp. 45-51, (2015) (PubMed).

Wang, Yang, Liu, Huang, Wang, Chen, Chen: "RBP-J-interacting and tubulin-associated protein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma by activating the p53-Fbxw7 pathway." in: Biochemical and biophysical research communications, Vol. 454, Issue 1, pp. 71-7, (2015) (PubMed).

Yang, Wang, Hui, Li, Jiang: "SOX 1, contrary to SOX 2, suppresses proliferation, migration, and invasion in human laryngeal squamous cell carcinoma by inhibiting the Wnt/?-catenin pathway." in: Tumour biology, (2015) (PubMed).

Shi, Lu, Zhou, Tan, He, Ding, Tian, Wang, Wang: "Long non-coding RNA Loc554202 regulates proliferation and migration in breast cancer cells." in: Biochemical and biophysical research communications, Vol. 446, Issue 2, pp. 448-53, (2014) (PubMed).

Ye, Li, Li, Yang, Niu, Wang: "Anti-tumor activity and relative mechanism of ethanolic extract of Marsdenia tenacissima (Asclepiadaceae) against human hematologic neoplasm in vitro and in vivo." in: Journal of ethnopharmacology, Vol. 153, Issue 1, pp. 258-67, (2014) (PubMed).

Lin, Wang, Wang, Fang, Zhang, Yang, Guo, Lin, Zhang, Wang: "Lithocarpus polystachyus REHD leaf aqueous extract inhibits human breast cancer growth in vitro and in vivo." in: Nutrition and cancer, Vol. 66, Issue 4, pp. 613-24, (2014) (PubMed).

He, Xiang, Zong, Yan, Yu, Liu, Zou, Yang: "CDK2-AP1 inhibits growth of breast cancer cells by regulating cell cycle and increasing docetaxel sensitivity in vivo and in vitro." in: Cancer cell international, Vol. 14, Issue 1, pp. 130, (2014) (PubMed).

Souza, Silva, Calloway, Kucuk, Rossi, McLemore: "Genistein protects hematopoietic stem cells against G-CSF-induced DNA damage." in: Cancer prevention research (Philadelphia, Pa.), Vol. 7, Issue 5, pp. 534-44, (2014) (PubMed).

Li, Yu, Liu, Zhu, He, Peng, Hua: "miR-34c works downstream of p53 leading to dairy goat male germline stem-cell (mGSCs) apoptosis." in: Cell proliferation, Vol. 46, Issue 2, pp. 223-31, (2013) (PubMed).

Teng, Chang, Wang, Lu, Wu, Yang, Chiu, Yang, Hsu, Ho: "Phospholipid-functionalized mesoporous silica nanocarriers for selective photodynamic therapy of cancer." in: Biomaterials, Vol. 34, Issue 30, pp. 7462-70, (2013) (PubMed).

Wang, Wu, Gao, Chen, Gu, Ding, Guo: "Elevated toll-like receptor 3 inhibits pancreatic ?-cell proliferation through G1 phase cell cycle arrest." in: Molecular and cellular endocrinology, Vol. 377, Issue 1-2, pp. 112-22, (2013) (PubMed).

Zhang, Tan, Wang, Chen, Quan, Xian, Zhang, He, Zhang: "Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway." in: Experimental cell research, Vol. 320, Issue 1, pp. 119-27, (2013) (PubMed).

Chen, Shi, Liu, Fang, Zhang, Zhao: "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." in: BMC cancer, Vol. 12, pp. 464, (2012) (PubMed).

Details zu Cyclin D1

Target: Cyclin D1 (CCND1)

Andere Bezeichnung: Cyclin D1

Hintergrund:

Synonyms: cD1, CycD1, Cyl-1, PRAD1, bcl-1, AI32739, G1/S-specific cyclin-D1, Ccnd1

Background: Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).

Gen-ID: 12443

UniProt: P25322

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, ER-Nucleus Signaling