Cyclin B1 Antikörper (AA 271-433)

Produktnummer ABIN670296

Dieses Anti-Cyclin B1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Cyclin B1 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) und IHC (fro). Geeignet für Human, Maus, Ratte, Rind (Kuh) und Huhn. Dieses Primary Antibody wurde in 8+ Publikationen zitiert.

Kurzübersicht für Cyclin B1 Antikörper (AA 271-433) (ABIN670296)

Target: Cyclin B1 (CCNB1)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Cyclin B1 Antikörper

Bindungsspezifität: AA 271-433

Kreuzreaktivität: Huhn, Rind (Kuh), Human, Maus, Ratte

Homologie: Cow

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CCNB1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-Cyclin B1 Antikörper (ABIN670296)

Wang, Wei, Xiao, Xue, Du, Liu, Xie: "Methamphetamine induces hepatotoxicity via inhibiting cell division, arresting cell cycle and activating apoptosis: In vivo and in vitro studies." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 105, pp. 61-72, (2017) (PubMed).

Ghate, Das, Chaudhuri, Panja, Mandal: "Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio." in: Cell death discovery, Vol. 2, pp. 15062, (2016) (PubMed).

Qu, Zou, Zhang, Sheng, Wang, Wang, Wang, Ji: "Chelidonine induces mitotic slippage and apoptotic-like death in SGC-7901 human gastric carcinoma cells." in: Molecular medicine reports, Vol. 13, Issue 2, pp. 1336-44, (2016) (PubMed).

Zhang, Pan, Xu, Niu, Ma, Xu: "Interleukin 18 augments growth ability via NF-?B and p38/ATF2 pathways by targeting cyclin B1, cyclin B2, cyclin A2, and Bcl-2 in BRL-3A rat liver cells." in: Gene, Vol. 563, Issue 1, pp. 45-51, (2015) (PubMed).

Wang, Wang: "Studying the relationship between cell cycle and Alzheimer's disease by gold nanoparticle probes." in: Analytical biochemistry, Vol. 489, pp. 32-7, (2015) (PubMed).

Guo, Cui, Peng, Fang, Zuo, Deng, Wang, Wu, Chen, Deng: "Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney." in: Oncotarget, Vol. 6, Issue 34, pp. 35964-77, (2015) (PubMed).

Dai, Ye, Peng, Zhou, Bai, Xiao, Ma, Zhou, Tang, Yao, Cao, Qin, Liu, Tan: "Aptamer TY04 inhibits the growth of multiple myeloma cells via cell cycle arrest." in: Tumour biology, Vol. 35, Issue 8, pp. 7561-8, (2014) (PubMed).

Ghate, Chaudhuri, Sarkar, Sajem, Panja, Rout, Mandal: "An antioxidant extract of tropical lichen, Parmotrema reticulatum, induces cell cycle arrest and apoptosis in breast carcinoma cell line MCF-7." in: PLoS ONE, Vol. 8, Issue 12, pp. e82293, (2013) (PubMed).

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: Cyclin B1

Hintergrund:

Synonyms: CycB1, Cycb-4, Cycb-5, Ccnb1-rs1, Cycb1-rs1, Ccnb1-rs13, G2/mitotic-specific cyclin-B1, Ccnb1, Ccn-2, Cycb

Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].

Gen-ID: 268697

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase