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MME Antikörper (AA 501-600)

Name: MME Antikörper (AA 501-600)
SKU: ABIN669666
Price: 357.7 EUR
Availability: InStock

Dieses Anti-MME-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MME in FACS, WB, ELISA, ICC, IHC (p), IHC (fro), IF (p) und IF (cc). Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Formalin-fixed and paraffin embedded mouse small intestine tissue labeled with Anti-CD10 Polyclonal Antibody (ABIN669666), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (MME Antikörper (AA 501-600))

IHC

Human Hela cells probed with CD10 Polyclonal Antibody, Alexa Fluor 647 conjugated -A647) (green) at 1:20 for 30 minutes compared to unstained cells (blue) and isotype control (orange). (MME Antikörper (AA 501-600))

FACS

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD10 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (MME Antikörper (AA 501-600))

IHC (p)

A549 cells probed with CD10 Polyclonal Antibody, Unconjugated at 1:20 for 30 minutes followed by incubation with a conjugated secondary -PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (MME Antikörper (AA 501-600))

FACS

5 Abbildungen

(1 reference)

Produktnummer ABIN669666

Datenblatt (PDF)

Kurzübersicht für MME Antikörper (AA 501-600) (ABIN669666)

Target

Alle MME Antikörper anzeigen

MME (Membrane Metallo-Endopeptidase (MME))

Reaktivität

Human, Maus, Ratte

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser MME Antikörper ist unkonjugiert

Applikation

Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Discover our top product MME Primärantikörper

Bindungsspezifität
49

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AA 501-600

Kreuzreaktivität

Human, Maus, Ratte

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human CD10

Isotyp

IgG
anti-Membrane Metallo-Endopeptidase (MME) (AA 52-352) antibody
MME Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 52-246) antibody
MME Reaktivität: Human WB, IHC, ELISA Wirt: Maus Monoclonal 7D4B1 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 382-538) antibody
MME Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 297-483) antibody
MME Reaktivität: Human WB, IHC, StM Wirt: Maus Monoclonal MME-1870 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 286-428) antibody
MME Reaktivität: Ratte WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Membrane Metallo-Endopeptidase (MME) antibody (FITC)
MME Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-78 FITC

anti-Membrane Metallo-Endopeptidase (MME) (AA 549-750) antibody
MME Reaktivität: Human FACS, IHC, ELISA Wirt: Maus Monoclonal 3G6B11 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 549-750) antibody
MME Reaktivität: Human FACS, WB, ELISA Wirt: Maus Monoclonal 6G12E12 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 297-483) antibody
MME Reaktivität: Human WB, IHC, ELISA, StM, Coat Wirt: Maus Monoclonal MME-1892 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 52-750) antibody
MME Reaktivität: Human, Ratte FACS, WB, IHC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Chopra, Kraus, Schwinn, Ritz, Mattenheimer, Mottok, Rosenwald, Einsele, Beilhack: "Non-invasive bioluminescence imaging to monitor the immunological control of a plasmablastic lymphoma-like B cell neoplasia after hematopoietic cell transplantation." in: PLoS ONE, Vol. 8, Issue 12, pp. e81320, (2013) (PubMed).
Target

MME (Membrane Metallo-Endopeptidase (MME))

Andere Bezeichnung

CD10

Substanzklasse

Chemical

Hintergrund

Synonyms: NEP, SFE, CD1, CALLA, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Neutral endopeptidase, Skin fibroblast elastase, MME, EPN
Background: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 3 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.

Gen-ID

4311

UniProt

P08473

Pathways

RTK Signalweg, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration