Glutamate Receptor 1 Antikörper (pSer845)

Produktnummer ABIN6658219

Hersteller Produkt- Nr.: 612-401-c83

Der Kaninchen Polyklonal Anti-Glutamate Receptor 1-Antikörper wurde für WB, IHC, ELISA und FM validiert. Er ist geeignet, Glutamate Receptor 1 in Proben von Ratte zu detektieren.

Kurzübersicht für Glutamate Receptor 1 Antikörper (pSer845) (ABIN6658219)

Target: Glutamate Receptor 1 (GLUR1)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glutamate Receptor 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Produktdetails anti-Glutamate Receptor 1 Antikörper

Bindungsspezifität: pSer845

Hersteller Produkt- Nr.: 612-401-c83

Hersteller: Rockland

Verwendungszweck: GluR1 phospho S845 Antibody

Kreuzreaktivität (Details): Anti-GluR1 pS845 Antibody is directed against GluR1 protein phosphorylated at S845.

Aufreinigung: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-GluR1 pS845 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:1000 - 10,000

Immunohistochemistry_Dilution: 1:1000

IF_Microscopy_Dilution: 1:200

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-GluR1 pS845 antibody is tested for use in IHC and Western Blotting and suitable for IF and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 100 kDa in size corresponding to GluR1 protein phosphorylated at Ser 845 in Western Blots of rat brain extracts.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Glutamate Receptor 1

Target: Glutamate Receptor 1 (GLUR1)

Andere Bezeichnung: GluR1

Hintergrund:

Synonyms: Glutamate receptor 1, GluR1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1,

Background: Anti-GluR1 pS845 antibody recognizes phophorylated GluR1. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR. In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory. Anti-GluR1 pS845 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: GRIA1

Gen-ID: 50592

NCBI Accession: NP_113796

UniProt: P19490

Pathways: PI3K-Akt Signalweg