Tryptophan Hydroxylase 1 Antikörper (pSer260)

Produktnummer ABIN6658146

Hersteller Produkt- Nr.: 600-401-e49

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Tryptophan Hydroxylase 1 in WB. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für Tryptophan Hydroxylase 1 Antikörper (pSer260) (ABIN6658146)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tryptophan Hydroxylase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Tryptophan Hydroxylase 1 Antikörper

Bindungsspezifität: pSer260

Hersteller Produkt- Nr.: 600-401-e49

Hersteller: Rockland

Verwendungszweck: Tryptophan Hydroxylase phospho S260 Antibody

Kreuzreaktivität (Details): Anti-Tryptophan Hydroxylase pS260 antibody is directed against rat Tryptophan Hydroxylase.

Aufreinigung: Tryptophan Hydroxylase pS260 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-Tryptophan Hydroxylase pS260 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 of Tryptophan Hydroxylase.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optional[Neutralization_Dilution]: 1:1000

Kommentare:

Anti-Tryptophan Hydroxylase pS260 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of 55 kDa in size corresponding to tryptophan hydroxylase protein phosphorylated at Ser260 in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Andere Bezeichnung: Tryptophan Hydroxylase

Hintergrund:

Synonyms: Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1, TPH

Background: Tryptophan Hydroxylase pS260 antibody detects Tryptophan Hydroxylase, which catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH. Anti-THPpS260 Antibody is ideal for investigators involved in Cell Signaling, and Neuroscience research.

Gene Name: TPH1

Gen-ID: 24848

UniProt: P09810

Pathways: Feeding Behaviour