HSP27 Antikörper (Internal Region)

Produktnummer ABIN6657932

Hersteller Produkt- Nr.: 200-301-f55

Dieses Anti-HSP27-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von HSP27 in WB, IHC, ELISA, IF, FACS, IP, MA und FM. Geeignet für Maus.

Kurzübersicht für HSP27 Antikörper (Internal Region) (ABIN6657932)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Klon: 8A7

Produktdetails anti-HSP27 Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 200-301-f55

Hersteller: Rockland

Verwendungszweck: Hsp25/Hsp27 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp25/27 from Mouse, Rat, Bovine, Canine, Guinea pig, and Hamster based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Hsp25/Hsp27 Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Hsp25/Hsp27 Antibody was produced in mice by repeated immunizations raised against an internal region of hsp27 synthetic peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: 1:200

Western_Blot_Dilution: 1:5000

Kommentare:

Anti-Hsp25/Hsp27 Antibody has been tested in WB, IHC, ICC/IF, IP, FC, and FACS. Expect a band approximately 25kDa or 27kDa protein corresponding to the molecular mass of Hsp25/27 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Andere Bezeichnung: Hsp25/Hsp27

Hintergrund:

Synonyms: HSPB2, Heat shock protein beta-2, HspB2, DMPK-binding protein, MKBP

Background: Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa. The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers. Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers. The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways. Other functions include chaperone activity (as mentioned above), thermo-tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin. And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth.

Gene Name: HSPB2

Gen-ID: 15507

NCBI Accession: NP_038588

UniProt: P14602

Pathways: MAPK Signalweg, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling