Ly6g Antikörper (APC)

Produktnummer ABIN6657868

Produktdetails anti-Ly6g Antikörper (APC)

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Ly6g Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity with Ly-6G (Gr-1) from other sources has not been tested

Aufreinigung: Allophycocyanin conjugated Ly-6G (Gr-1) Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse Ly-6G (Gr-1). . Anti-Ly-6G (Gr-1) is conjugated with APC under optimal conditions and the solution is free of unconjugated APC. Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C.

Immunogen:

Immunogen: Anti-Ly-6G (Gr-1) Antibody (Monoclonal) was produced by repeated immunizations with Ly-6G/Ly-6C (Gr-1) antigen.

Klon: RB6-8C5

Isotyp: IgG2b

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Ly-6G (Gr-1) is useful for Immunohistochemistry, Immunoprecipitation, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Immunoprecipitation Dilution: User Optimized

Flow Cytometry Dilution: 10 μL/10^6 cells (0.1 μg)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Details zu Ly6g

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Andere Bezeichnung: Ly-6G (Gr-1) (Ly6g Produkte)

Synonyme: Gr-1 antikoerper, Gr1 antikoerper, Ly-6G antikoerper, lymphocyte antigen 6 complex, locus G antikoerper, Ly6g antikoerper

Hintergrund:

Synonyms: Ly-6G/Ly-6C (Gr-1), RB6-8C5, Gr-1, Ly-6G, Ly-6C

Background: The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.

Gene Name: Gr-1

Gen-ID: 17067

NCBI Accession: NP_001238984