Nitrotyrosine Antikörper

Produktnummer ABIN6657486

Hersteller Produkt- Nr.: 200-301-a98

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Nitrotyrosine in WB, ELISA und IHC. Er zeigt eine Reaktivität gegenüber .

Kurzübersicht für Nitrotyrosine Antikörper (ABIN6657486)

Target: Nitrotyrosine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nitrotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Klon: 39B6

Produktdetails anti-Nitrotyrosine Antikörper

Hersteller Produkt- Nr.: 200-301-a98

Hersteller: Rockland

Verwendungszweck: Nitrotyrosine Antibody

Kreuzreaktivität (Details): This antibody is known to react with nitrated tyrosine residues from human, mouse, rat and dog sources.

Aufreinigung: This Protein G purified monoclonal antibody reacts with proteins containing 3-nitrotyrosine moieties and does not detect non-nitrated tyrosine residues.

Sterilität: Sterile filtered

Immunogen: This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid - BSA conjugate.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:200 - 1:1,000

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Kommentare:

This Protein G purified antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Approximately 0.7 μg of antibody is sufficient to detect 5 μg of SIN-1 treated BSA by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Nitrotyrosine

Target: Nitrotyrosine

Substanzklasse: Chemical

Hintergrund:

Background: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation-induced tissue injury (1). The presence of nitro tyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Gene Name: n/a