TARDBP Antikörper (Cleavage Site)

Produktnummer ABIN6657361

Hersteller Produkt- Nr.: 600-401-e43

Der Kaninchen Polyklonal Anti-TARDBP-Antikörper wurde für WB und IHC validiert. Er ist geeignet, TARDBP in Proben von Human zu detektieren.

Kurzübersicht für TARDBP Antikörper (Cleavage Site) (ABIN6657361)

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TARDBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-TARDBP Antikörper

Bindungsspezifität: Cleavage Site

Hersteller Produkt- Nr.: 600-401-e43

Hersteller: Rockland

Verwendungszweck: TDP43 Antibody

Kreuzreaktivität (Details): TDP43 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification.

Aufreinigung: Anti-TDP43 antibody is directed against human TDP43.

Immunogen: Anti-TDP43 Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues C-terminal to the caspase-cleavage site (between D219 and V220) of the TAR DNA-binding protein 43.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 1:250

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-TDP43 (Rabbit) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 43 kDa in size corresponding to the TDP43 protein in Western Blots of rat brain lysate. Molecular weight is dependent upon the TDP43 species present in the sample, full-length or truncated TDP43. Under non-denaturing conditions (for example, by IHC), this antibody detects TDP-43 inclusions in human brain tissue with TDP-43 proteinopathy, but does not detect full-length nuclear TDP-43. Under denaturing conditions (for example, by Western Blot analysis) this antibody detects the C-terminus of full-length and truncated human TDP-43. Researchers should determine optimal titers for applications that are not stated below.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu TARDBP

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Andere Bezeichnung: TDP43

Hintergrund:

Synonyms: TAR DNA-binding protein 43

Background: TDP43 antibody detects TDP43 protein (Tar DNA Binding 43, TARDBP). TDP43 was originally identified as a protein that binds to the "transactivation response" (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome. UV cross-linking of HeLa cell extract revealed a 43 kDa protein which was cloned and sequenced and shown to contain two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis. The protein is present in these inclusions in partially degraded, hyperphosphorylated and ubiquitinated forms. Anti-TDP43 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: TARDBP

Gen-ID: 23435

UniProt: Q13148

Pathways: Positive Regulation of Peptide Hormone Secretion