GFAP Antikörper (AA 411-422)

Produktnummer ABIN6656791

Hersteller Produkt- Nr.: 200-301-w55

Der Maus Monoklonal Anti-GFAP-Antikörper wurde für WB, IHC, FM und MA validiert. Er ist geeignet, GFAP in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für GFAP Antikörper (AA 411-422) (ABIN6656791)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Klon: S206A-8

Produktdetails anti-GFAP Antikörper

Bindungsspezifität: AA 411-422

Hersteller Produkt- Nr.: 200-301-w55

Hersteller: Rockland

Verwendungszweck: GFAP Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with rat and mouse based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-GFAP Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Anti-GFAP Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids 411-422 (KTVEMRDGEVIK) of human GFAP.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-GFAP Antibody is tested for use in Western blot, Immunohistochemistry, and Immunocytochemistry. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-GFAP Antikörper (ABIN6656791)

Rodrigues-Amorim, Rivera-Baltanás, Del Carmen Vallejo-Curto, Rodriguez-Jamardo, de Las Heras, Barreiro-Villar, Blanco-Formoso, Fernández-Palleiro, Álvarez-Ariza, López, García-Caballero, Olivares, Spuch: "Plasma β-III tubulin, neurofilament light chain and glial fibrillary acidic protein are associated with neurodegeneration and progression in schizophrenia." in: Scientific reports, Vol. 10, Issue 1, pp. 14271, (2021) (PubMed).

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP

Hintergrund:

Synonyms: Glial fibrillary acidic protein, Intermediate filament protein, Astrocyte, gfapl, DKFZp459C0729, MGC139638, FLJ45472, AI836096, GFAP antibody

Background: The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia. Anti-GFAP is ideal for investigators involved in Neuroscience Research, including Alexander Disease, Oligodendroglioma, Cytoskleton Remolding Neurofilaments and PIP3/AKT Signaling.

Gene Name: GFAP

Gen-ID: 2670

UniProt: P14136