ATM Antikörper (pSer1981) (Biotin)

Produktnummer ABIN6656105

Hersteller Produkt- Nr.: 200-306-400

Dieses Anti-ATM-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ATM in WB, IHC, ELISA und FM. Geeignet für Human.

Kurzübersicht für ATM Antikörper (pSer1981) (Biotin) (ABIN6656105)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATM Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Klon: 10H11-E12

Produktdetails anti-ATM Antikörper (Biotin)

Bindungsspezifität: AA 1974-1988, pSer1981

Hersteller Produkt- Nr.: 200-306-400

Hersteller: Rockland

Verwendungszweck: ATM phospho S1981 Biotin Conjugated Antibody

Kreuzreaktivität (Details): This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts.

Aufreinigung: This Protein A Purified Mab antibody is directed against human ATM and is useful in determining its presence in various assays.

Immunogen: This antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:20,000 - 1:100,000

Immunohistochemistry_Dilution: 1:1,000 - 1:5,000

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Kommentare:

This antibody was tested by ELISA and western blotting against both the native and recombinant forms of the protein. This reagent may also be suitable other biotin-streptavidin based assays.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Andere Bezeichnung: ATM

Hintergrund:

Synonyms: mouse anti-ATM antibody biotin, mouse anti-ATMpS1981 antibody biotin conjugation, biotin conjugated mouse anti- ATM pS1981 antibody, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody, AT mutated antibody, AT protein antibody, AT1 antibody, ATA antibody, Ataxia telangiectasia gene mutated in human beings antibody, Ataxia telangiectasia mutated antibody, ATC antibody, ATDC antibody, ATE antibody, ATM antibody

Background: ATM, the gene mutated in the hereditary disease ataxia-telangiectasia, codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure.  The ATM gene encodes a 370- kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint, Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest, and Brca1 and hRad17 in the G2/M checkpoint. Ideal for Cancer, Cell Signaling, Chromatin, Neuroscience and Signal Transduction research.

Gene Name: ATM

Gen-ID: 472

NCBI Accession: NP_000042

UniProt: Q13315

Pathways: p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus