ATM Antikörper (pSer1981)

Produktnummer ABIN6655681

Hersteller Produkt- Nr.: 200-301-500

Der Maus Monoklonal Anti-ATM-Antikörper wurde für WB, IHC, ELISA und FACS validiert. Er ist geeignet, ATM in Proben von Human zu detektieren. Es sind 27+ Publikationen verfügbar.

Kurzübersicht für ATM Antikörper (pSer1981) (ABIN6655681)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Klon: 7C10D8

Produktdetails anti-ATM Antikörper

Bindungsspezifität: AA 1974-1988, pSer1981

Hersteller Produkt- Nr.: 200-301-500

Hersteller: Rockland

Verwendungszweck: ATM phospho S1981 Antibody

Kreuzreaktivität (Details): This monoclonal anti-ATM antibody recognizes the phosphorylated form of the protein in native and over-expressed proteins found in various tissues and extracts.

Aufreinigung: This Protein A Purified Mab antibody is directed against human ATM and is useful in determining its presence by immunohistochemistry.

Sterilität: Sterile filtered

Immunogen: This antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100 - 1:500

Flow_Cytometry_Dilution: User Optimized

Western_Blot_Dilution: 1:500- 1:2,000

Other: User Optimized

Kommentare:

Suggested Applications: IF, IP, Multiplex, WB
Anti-ATM Protein Kinase pS1981 (MOUSE) Monoclonal Antibody clone has been tested by ELISA and optimized for IHC, but may also be suitable for Western blot, flow cytometry, immunoprecipitation, and immunofluorescence microscopy. Indirect immunoperoxidase staining on formaldehyde-fixed, de-paraffinized tissue sections and/or formalin-fixed cultured cells are recommended when using this antibody as described in Bartkova et al 2005.  Product item# 200-301-400 produced from clone 10H11.E12 has been optimized for WB, IP and IF.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-ATM Antikörper (ABIN6655681)

Jiang, Li, Schroer, Voisin, Ju, Pacal, Erdmann, Shi, Chung, Deng, Chen, Ciavarra, Datti, Mak, Harrington, Dick, Bader, Bremner, Woo, Zacksenhaus: "Hypophosphorylated pRb knock-in mice exhibit hallmarks of aging and vitamin C-preventable diabetes." in: The EMBO journal, Vol. 41, Issue 4, pp. e106825, (2022) (PubMed).

Mattiello, Pucci, Marchetti, Diederich, Gonfloni: "Asciminib Mitigates DNA Damage Stress Signaling Induced by Cyclophosphamide in the Ovary." in: International journal of molecular sciences, Vol. 22, Issue 3, (2021) (PubMed).

Sakai, Takagaki, Yamagiwa, Ui, Hatta, Imai: "Effects of the Cytoplasm and Mitochondrial Specific Hydroxyl Radical Scavengers TA293 and mitoTA293 in Bleomycin-Induced Pulmonary Fibrosis Model Mice." in: Antioxidants (Basel, Switzerland), Vol. 10, Issue 9, (2021) (PubMed).

Bellusci, Mattiello, Iannizzotto, Ciccone, Maiani, Villani, Diederich, Gonfloni: "Kinase-independent inhibition of cyclophosphamide-induced pathways protects the ovarian reserve and prolongs fertility." in: Cell death & disease, Vol. 10, Issue 10, pp. 726, (2020) (PubMed).

Rosina, Langone, Giuliani, Cerquone Perpetuini, Reggio, Calderone, Fuoco, Castagnoli, Gargioli, Cesareni: "Osteogenic differentiation of skeletal muscle progenitor cells is activated by the DNA damage response." in: Scientific reports, Vol. 9, Issue 1, pp. 5447, (2019) (PubMed).

Nagane, Kuppusamy, An, Mast, Gogna, Yasui, Yamamori, Inanami, Kuppusamy: "Ataxia-Telangiectasia Mutated (ATM) Kinase Regulates eNOS Expression and Modulates Radiosensitivity in Endothelial Cells Exposed to Ionizing Radiation." in: Radiation research, Vol. 189, Issue 5, pp. 519-528, (2018) (PubMed).

Kim, Xia, Suh, Lee, Jun, Lien, Zhang, Chen, Park: "PAF-Myc-Controlled Cell Stemness Is Required for Intestinal Regeneration and Tumorigenesis." in: Developmental cell, Vol. 44, Issue 5, pp. 582-596.e4, (2018) (PubMed).

Emery, Gopalan, Wood, Chow, Battelli, George, Blaszyk, Florman, Yun: "Expression and function of ABCG2 and XIAP in glioblastomas." in: Journal of neuro-oncology, Vol. 133, Issue 1, pp. 47-57, (2018) (PubMed).

Sinha, Qin, Li: "A p53/ARF-dependent anticancer barrier activates senescence and blocks tumorigenesis without impacting apoptosis." in: Molecular cancer research : MCR, Vol. 13, Issue 2, pp. 231-8, (2016) (PubMed).

Nagane, Yasui, Sakai, Yamamori, Niwa, Hattori, Kondo, Inanami: "Activation of eNOS in endothelial cells exposed to ionizing radiation involves components of the DNA damage response pathway." in: Biochemical and biophysical research communications, Vol. 456, Issue 1, pp. 541-6, (2015) (PubMed).

Liu, Xu, OPrey, Lao, Joshi, Long, OPrey, Croft, Beaumatin, Baudot, Mrschtik, Rosenfeldt, Zhang, Gillespie, Ryan: "Loss of autophagy causes a synthetic lethal deficiency in DNA repair." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 3, pp. 773-8, (2015) (PubMed).

Chene, Ouellet, Rahimi, Barres, Caceres, Meunier, Cyr, De Ladurantaye, Provencher, Mes Masson: "DNA damage signaling and apoptosis in preinvasive tubal lesions of ovarian carcinoma." in: International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, Vol. 25, Issue 5, pp. 761-9, (2015) (PubMed).

Phesse, Myant, Cole, Ridgway, Pearson, Muncan, van den Brink, Vousden, Sears, Vassilev, Clarke, Sansom: "Endogenous c-Myc is essential for p53-induced apoptosis in response to DNA damage in vivo." in: Cell death and differentiation, Vol. 21, Issue 6, pp. 956-66, (2014) (PubMed).

Broering, Alavattam, Sadreyev, Ichijima, Kato, Hasegawa, Camerini-Otero, Lee, Andreassen, Namekawa: "BRCA1 establishes DNA damage signaling and pericentric heterochromatin of the X chromosome in male meiosis." in: The Journal of cell biology, Vol. 205, Issue 5, pp. 663-75, (2014) (PubMed).

Vidal-Eychenié, Décaillet, Basbous, Constantinou: "DNA structure-specific priming of ATR activation by DNA-PKcs." in: The Journal of cell biology, Vol. 202, Issue 3, pp. 421-9, (2013) (PubMed).

Shamma, Suzuki, Hayashi, Kobayashi, Sasaki, Nishiuchi, Doki, Okamoto, Kohno, Muranaka, Kitajima, Yamamoto, Takahashi: "ATM mediates pRB function to control DNMT1 protein stability and DNA methylation." in: Molecular and cellular biology, Vol. 33, Issue 16, pp. 3113-24, (2013) (PubMed).

Parikh, Shuck, Nguyen, Herron, Donehower: "Mouse tissues that undergo neoplastic progression after K-Ras activation are distinguished by nuclear translocation of phospho-Erk1/2 and robust tumor suppressor responses." in: Molecular cancer research : MCR, Vol. 10, Issue 6, pp. 845-55, (2012) (PubMed).

Schwab, Smith, Dressler: "Arrested spermatogenesis and evidence for DNA damage in PTIP mutant testes." in: Developmental biology, Vol. 373, Issue 1, pp. 64-71, (2012) (PubMed).

Raynaud, Hernandez, Llorca, Nuciforo, Mathieu, Commo, Delaloge, Sabatier, André, Soria: "DNA damage repair and telomere length in normal breast, preneoplastic lesions, and invasive cancer." in: American journal of clinical oncology, Vol. 33, Issue 4, pp. 341-5, (2010) (PubMed).

Lantuejoul, Raynaud, Salameire, Gazzeri, Moro-Sibilot, Soria, Brambilla, Brambilla: "Telomere maintenance and DNA damage responses during lung carcinogenesis." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 11, pp. 2979-88, (2010) (PubMed).

Details zu ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Andere Bezeichnung: ATM

Hintergrund:

Synonyms: mouse anti-ATM antibody, mouse anti-ATMpS1981 antibody, mouse anti- ATM pS1981 antibody, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody

Background: Anti ATM pS1981 Antibody recognizes the product of the ATM gene that is mutated in the hereditary disease ataxia-telangiectasia. ATM codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure.  The ATM gene encodes a 370- kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint, Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest, and Brca1 and hRad17 in the G2/M checkpoint. See Bakkenist, C. J. & Kastan, M. B. Nature 421, 499-506 (2003) for a complete presentation of this antibody's specificity and utility.

Gene Name: ATM

Gen-ID: 472

NCBI Accession: NP_000042

UniProt: Q13315

Pathways: p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus