CD74 Antikörper

Produktnummer ABIN6655211

Hersteller Produkt- Nr.: 209-301-f12

Dieses Anti-CD74-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD74 in FACS, WB, IHC und IP. Geeignet für Human.

Kurzübersicht für CD74 Antikörper (ABIN6655211)

Target: CD74 (HLA-DR-gamma (CD74))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD74 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Klon: PIN-1

Produktdetails anti-CD74 Antikörper

Hersteller Produkt- Nr.: 209-301-f12

Hersteller: Rockland

Verwendungszweck: CD74 Antibody

Kreuzreaktivität (Details): This monoclonal antibody is specific for human CD74.

Aufreinigung: Anti-CD74 Antibody was immunoglobulin purified.

Sterilität: Sterile filtered

Immunogen: CD74 Antibody was produced in mice by repeated immunizations with a human CD74 invariant chain synthetic peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: User Optimized

Western_Blot_Dilution: 1 μg/mL

Kommentare:

Anti-CD74 Antibody is tested for WB and suitable for IP, Flow Cytometry, FACS, and IHC. Expect a band approximately ~33-35kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu CD74

Target: CD74 (HLA-DR-gamma (CD74))

Andere Bezeichnung: CD74

Hintergrund:

Synonyms: mouse anti-CD74 antibody, mouse anti-Cluster of Differentiation 74 antibody, DHLAG, HLA DR gamma, HLADG, p33, p35, Protein 41 antibody, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain

Background: CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain. The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC), however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules. Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases.

Gene Name: CD74

Gen-ID: 972

NCBI Accession: NP_001020329

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints