MAP2 Antikörper

Produktnummer ABIN6655044

Hersteller Produkt- Nr.: 200-901-d68

Produktdetails anti-MAP2 Antikörper

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reaktivität: Rind (Kuh)

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser MAP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 200-901-d68

Hersteller: Rockland

Verwendungszweck: Microtubule Associated Protein 2 (MAP2) Antibody

Kreuzreaktivität (Details): Anti-MAP2 antibody is directed against MAP2 protein.

Aufreinigung: The antibody is a total IgY fraction.

Immunogen: Anti-Microtubule Associated Protein 2 Antibody was produced in chicken by repeated immunizations with recombinant bovine MAP2 protein expressed in and purified from E. Coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IF_Microscopy_Dilution: 1:2500

Western_Blot_Dilution: 1:20000

Other: User Optimized

Kommentare:

Anti-Microtubule Associated Protein 2 antibody is tested for use in Western Blotting, ICC, and IHC. Specific conditions should be optimized by the end user. Expect a band of approximately 280 kDa in size corresponding to MAP2 proteins in Western blots in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Andere Bezeichnung: Microtubule Associated Protein 2 (MAP2) (MAP2 Produkte)

Hintergrund:

Synonyms: Microtubule-associated protein 2, MAP2, MAP-2,Map2, Map-2

Background: MAP2 antibody detects Microtubule Associated Protein 2. Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone.

Gene Name: MAP2

Gen-ID: 4133

UniProt: P11137