Cyclin E1 Antikörper (AA 10-176)

Produktnummer ABIN6654284

Dieses Anti-Cyclin E1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cyclin E1 in IHC (p). Geeignet für Human.

Kurzübersicht für Cyclin E1 Antikörper (AA 10-176) (ABIN6654284)

Target: Cyclin E1 (CCNE1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin E1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: CCNE1-2460

Produktdetails anti-Cyclin E1 Antikörper

Bindungsspezifität: AA 10-176

Aufreinigung: Purified

Reinheit: Protein G affinity chromatography

Immunogen: Recombinant human partial protein (within amino acids 10-176) was used as the immunogen for the Cyclin E1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Cyclin E1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Cyclin E1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Cyclin E1

Target: Cyclin E1 (CCNE1)

Andere Bezeichnung: Cyclin E1 / CCNE1

Hintergrund: Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases