APEX Nuclease (Multifunctional DNA Repair Enzyme) 1 (APEX1) Antikörper

Details zu Produkt Nr. ABIN6569299
Target
Reaktivität
Human
107
44
41
9
9
7
7
6
6
5
4
4
2
1
1
1
1
1
1
Wirt
Kaninchen
81
29
3
Klonalität
Polyklonal
Konjugat
Unkonjugiert
4
4
4
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
92
37
35
34
21
13
12
9
9
6
5
3
2
2
1
1
Optionen
Immunogen Recombinant protein of human APEX1
Isotyp IgG
Reinigung Affinity purification
Target
Andere Bezeichnung APEX1 (APEX1 Antibody Abstract)
Hintergrund

Synonyms: AP endonuclease 1,AP endonuclease class I,AP lyase,APE 1,APE,APE-1,APEN,APEX 1,APEX,APEX nuclease (multifunctional DNA repair enzyme) 1,Apex nuclease 1,APEX nuclease,APEX1,APEX1,Apurinic endonuclease,Apurinic-apyrimidinic endonuclease 1,Apurinic/apyrimidinic (abasic) endonuclease,Apurinic/apyrimidinic endonuclease 1,Apurinic/apyrimidinic exonuclease,APX,BAP1,Deoxyribonuclease (apurinic or apyrimidinic),DNA (apurinic or apyrimidinic site) lyase,DNA-(apurinic or apyrimidinic site) lyase,mitochondrial,EC 4.2.99.18,HAP 1,HAP1,Human Apurinic endonuclease 1,MGC139790,Multifunctional DNA repair enzyme,Redox factor 1,Redox factor-1,REF 1,REF 1 protein,REF-1,REF1,REF1 protein

Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene, all encode the same protein.

Molekulargewicht

Observed_MW: 40kDa

Calculated_MW: 35kDa

Gen-ID 328
UniProt P27695
Pathways DNA Reparatur, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
Applikations-hinweise WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1 mg/mL
Buffer PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C. Avoid freeze / thaw cycles.
Bilder
Image no. 1 for anti-APEX Nuclease (Multifunctional DNA Repair Enzyme) 1 (APEX1) antibody (ABIN6569299) Western blot analysis of extracts of various cell lines, using APEX1 antibody.
Image no. 2 for anti-APEX Nuclease (Multifunctional DNA Repair Enzyme) 1 (APEX1) antibody (ABIN6569299) Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug APEX1 antibody.
Image no. 3 for anti-APEX Nuclease (Multifunctional DNA Repair Enzyme) 1 (APEX1) antibody (ABIN6569299) Immunofluorescence analysis of HeLa cells using APEX1 antibody.
Image no. 4 for anti-APEX Nuclease (Multifunctional DNA Repair Enzyme) 1 (APEX1) antibody (ABIN6569299) Immunofluorescence analysis of A549 cells using APEX1 antibody.