DFFA Antikörper (DNA Fragmentation Factor, 45kDa, alpha Polypeptide)

Details for Product anti-DFFA Antibody No. ABIN6566873
Target
Reaktivität
Human, Maus, Ratte
96
45
32
2
1
1
1
1
Wirt
Kaninchen
111
13
1
Klonalität
Polyklonal
Konjugat
Dieser DFFA Antikörper ist unkonjugiert
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Applikation
Immunofluorescence (IF), Western Blotting (WB)
100
45
28
19
19
18
13
9
6
3
2
2
1
1
1
1
Optionen
Immunogen Recombinant protein of human DFFA
Isotyp IgG
Reinigung Affinity purification
Target
Andere Bezeichnung DFFA (DFFA Antibody Abstract)
Hintergrund

Synonyms: A330085O09Rik,Caspase activated deoxyribonuclease inhibitor short form,DFF 1,DFF 45,DFF alpha,DFF-45,DFF1,DFF35,DFF45,DFFA,Dffa DNA fragmentation factor,alpha subunit,DFFA,DNA fragmentation factor 45 kDa subunit,DNA Fragmentation Factor Alpha Subunit,DNA fragmentation factor subunit alpha,DNA fragmentation factor,45 kD,alpha subunit,DNA fragmentation factor,45  kDa,alpha polypeptide (DFFA),transcript variant 1,DNA fragmentation factor,45  kDa,alpha polypeptide,DNA fragmentation factor,alpha subunit,DNAation factor 45 kDa subunit,H13,ICAD,ICAD L,ICAD S,Inhibitor of CAD,Inhibitor of Caspase Activated DNase,MGC143066,OTTHUMP00000001903,OTTHUMP00000001904,RP23 121D17.3

Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Molekulargewicht

Observed_MW: 35kDa, 45kDa

Calculated_MW: 29kDa/36kDa

Gen-ID 1676
UniProt O00273
Pathways Apoptose, Caspase Kaskade in der Apoptose
Applikations-hinweise WB 1:500 - 1:2000 IF 1:50 - 1:100
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1 mg/mL
Buffer Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C. Avoid freeze / thaw cycles.
Bilder
Image no. 1 for anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody (ABIN6566873) Western blot analysis of extracts of various cell lines, using DFFA antibody.
Image no. 2 for anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody (ABIN6566873) Immunofluorescence analysis of HeLa cells using DFFA antibody.