MGMT Antikörper (AA 8-221)

Produktnummer ABIN6560107

Produktdetails anti-MGMT Antikörper

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Bindungsspezifität: AA 8-221

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MGMT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Species Reactivity: Human. Cross-reacts weakly with mouse, rat and hamster. Other species not tested. Molecular Weight: .25 kDa Cellular Location: Nuclear and cytoplasmic.Positive Control: Raji cells, Colon carcinoma.

Immunogen: Purified recombinant human MGMT protein.

Klon: MT3-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry Immunohistochemistry: (formalin / paraffin) Use at a 1:20 - 1:40 dilution for 60 minutes at room temperature (RT). Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10 - 20 minutes followed by cooling at RT for 20 minutes. Note: IHC requires the use of a high-efficiency detection system. Western blot: Use at 0.5-1 µg/mL for 2 hours at RT. The optimal dilution for a specific application should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 500 µL of monoclonal antibody at 200 µg/mL in 10 mM PBS, pH 7.4, with protein stabilizer and 0.09% sodium azide. Purified from ascites fluid by Protein G chromatography.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Andere Bezeichnung: MGMT (MGMT Produkte)

Synonyme: AGT antikoerper, AI267024 antikoerper, Agat antikoerper, O-6-methylguanine-DNA methyltransferase antikoerper, MGMT antikoerper, Mgmt antikoerper

Hintergrund: Cancer chemotherapeutic alkylating agents (e.g. BCNU) act by inducing formation of lethal cross-links at the O6-alkylguanine position in DNA. MGMT (O6-methylguanine-DNA methyltransfer-ase) transfers alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA-damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug-resistant.

Pathways: DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus