Complement C4d Antikörper

Produktnummer ABIN640791

Der Maus Monoklonal anti-Complement C4d Antikörper (ABIN640791) detektiert spezifisch Complement C4d in ELISA, IF, WB, IHC (p) und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Complement C4d Antikörper (ABIN640791)

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Complement C4d Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Complement C4d Antikörper

Verwendungszweck: Mouse Anti-Human C4d

Spezifität: DS-MB-00163 recognizes the secreted protein complement component 4d (C4d). The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b's alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4d's short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. C4 has been linked to susceptibility to systemic lupus erythematosous. ImmunohistologyIt has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomeruli and in some cases the peritubular capillaries. DS-MB-00163 has given variable results on formalin-fixed, paraffin-embedded sections. It has been observed that pre-treatment with 88% formic acid for 20 minutes at room temperature is beneficial (6).

Produktmerkmale: Blood Coagulation Proteins

Aufreinigung: Purified

Immunogen: Native, from human plasma

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology - Frozen, ELISA (dilution: 1/250, 1/600), Immunofluorescence, Immunohistology - Paraffin, Western Blotting

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: IgG concentration 1.0 mg/ml

Buffer: Borate buffered saline pH 8.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu Complement C4d

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Andere Bezeichnung: C4d