HNF4A Antikörper (Internal Region)

Produktnummer ABIN6391399

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch HNF4A in ELISA und IF. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für HNF4A Antikörper (Internal Region) (ABIN6391399)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HNF4A Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-HNF4A Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: HNF4A

Sequenz: HPHLMQEHMG TN

Spezifität: This antibody is expected to recognise isoforms 1, 2, 4, 5, 7, 8 and 9

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary experiments showed an approx 55 kDa band in Human Colon, Duodenum, Kidney and Liver lysates (calculated MW of 52.8 kDa according to NP_000448.3). Recommended concentration:0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Andere Bezeichnung: HNF4A

Hintergrund: HNF4A, hepatocyte nuclear factor 4, alpha , FLJ39654, HNF4, HNF4a7, HNF4a8, HNF4a9, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14, HNF4-alpha, hepatic nuclear factor 4 alpha, hepatocyte nuclear factor 4 alpha, transcription factor-14

Gen-ID: 3172

NCBI Accession: NP_849180, NP_000448, NP_787110, NP_001025174, NP_001245284, NP_001274111, NP_001274112

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process