HNF4A Antikörper (Internal Region)

Produktnummer ABIN6391399

Dieser Ziege Polyklonal Antikörper detektiert spezifisch HNF4A in ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für HNF4A Antikörper (Internal Region) (ABIN6391399)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HNF4A Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-HNF4A Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: HNF4A

Sequenz: HPHLMQEHMG TN

Spezifität: This antibody is expected to recognise isoforms 1, 2, 4, 5, 7, 8 and 9

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary experiments showed an approx 55 kDa band in Human Colon, Duodenum, Kidney and Liver lysates (calculated MW of 52.8 kDa according to NP_000448.3). Recommended concentration:0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Andere Bezeichnung: HNF4A

Hintergrund: HNF4A, hepatocyte nuclear factor 4, alpha , FLJ39654, HNF4, HNF4a7, HNF4a8, HNF4a9, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14, HNF4-alpha, hepatic nuclear factor 4 alpha, hepatocyte nuclear factor 4 alpha, transcription factor-14

Gen-ID: 3172

NCBI Accession: NP_849180, NP_000448, NP_787110, NP_001025174, NP_001245284, NP_001274111, NP_001274112

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process