Fibrillarin Antikörper (N-Term)

Produktnummer ABIN629933

Der Kaninchen Polyklonal Anti-Fibrillarin-Antikörper wurde für WB und IHC validiert. Er ist geeignet, Fibrillarin in Proben von Human, Ratte, Maus und Hund zu detektieren.

Kurzübersicht für Fibrillarin Antikörper (N-Term) (ABIN629933)

Target: Fibrillarin (FBL)

Reaktivität: Human, Ratte, Maus, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Fibrillarin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Fibrillarin Antikörper

Bindungsspezifität: N-Term

Spezifität: Fibrillarin antibody was raised against the N terminal of FBL

Aufreinigung: Purified

Immunogen: Fibrillarin antibody was raised using the N terminal of FBL corresponding to a region with amino acids GGGFHSGGNRGRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKN

Anwendungsinformationen

Applikationshinweise: WB: 1.25 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator.

Kommentare:

Fibrillarin Blocking Peptide, (ABIN938665), is also available for use as a blocking control in assays to test for specificity of this Fibrillarin antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of FBL antibody in PBS

Konzentration: Lot specific

Buffer: PBS

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Details zu Fibrillarin

Target: Fibrillarin (FBL)

Andere Bezeichnung: Fibrillarin

Hintergrund: FBL is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. FBL contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognise fibrillarin.

Molekulargewicht: 35 kDa (MW of target protein)

Pathways: Ribonucleoside Biosynthetic Process