Major Capsid Protein L1 Antikörper

Produktnummer ABIN6296477

Der Maus Monoklonal Anti--Antikörper wurde für WB, ELISA, IHC und IF validiert. Er ist geeignet, in Proben von Human Papillomavirus 16 (HPV-16) zu detektieren.

Kurzübersicht für Major Capsid Protein L1 Antikörper (ABIN6296477)

Target: Major Capsid Protein L1

Reaktivität: Human Papillomavirus 16 (HPV-16)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails

Verwendungszweck: Mouse anti-Type 16 of Human Papilloma Virus (HPV-16) L1 Antibody [Sodium Azide Free]

Spezifität: Nuclear

Aufreinigung: Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the L1 antibody.

Immunogen: Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the L1 antibody.

Anwendungsinformationen

Applikationshinweise: Western blot: 1-2 μg/mL,ELISA: assay dependent
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Antigendetails

Target: Major Capsid Protein L1

Substanzklasse: Viral Protein

Hintergrund:

Target Description: Reacts with a protein of 57 kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Basement membrane is exposed only after epithelium trauma. Additionally, the alpha6 integrin complexed with either beta1 or beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, integrin complexed with beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2. [UniProt]The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33, very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.

Gene Symbol: L1

UniProt: P03101