Cyclin B1 Antikörper

Produktnummer ABIN6296320

Dieser Maus Monoklonal Antikörper detektiert spezifisch Cyclin B1 in IF, FACS, IP und BP. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Hamster.

Kurzübersicht für Cyclin B1 Antikörper (ABIN6296320)

Target: Cyclin B1 (CCNB1)

Reaktivität: Human, Maus, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Peptide (BP)

Produktdetails anti-Cyclin B1 Antikörper

Verwendungszweck: Mouse anti-Human/Mouse/Hamster Cyclin B1 Antibody [Sodium Azide Free]

Spezifität: Cytoplasmic

Aufreinigung: Hamster protein was used as the immunogen for this Cyclin B1 antibody.

Immunogen: Hamster protein was used as the immunogen for this Cyclin B1 antibody.

Anwendungsinformationen

Applikationshinweise: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IP: 1-2 μg/500 μg protein--precipitates active Cyclin B1 complexes

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: G2/mitotic cyclin-B1

Hintergrund:

Target Description: This antibody recognizes a protein of 55-62 kDa, identified as Cyclin B1. In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.

Gene Symbol: CCNB1///CCNB

Gen-ID: 891

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase