Cyclin B1 Antikörper

Produktnummer ABIN6296320

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cyclin B1 in IF, FACS, IP und BP. Er zeigt eine Reaktivität gegenüber Human, Maus und Hamster.

Kurzübersicht für Cyclin B1 Antikörper (ABIN6296320)

Target: Cyclin B1 (CCNB1)

Reaktivität: Human, Maus, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Peptide (BP)

Produktdetails anti-Cyclin B1 Antikörper

Verwendungszweck: Mouse anti-Human/Mouse/Hamster Cyclin B1 Antibody [Sodium Azide Free]

Spezifität: Cytoplasmic

Aufreinigung: Hamster protein was used as the immunogen for this Cyclin B1 antibody.

Immunogen: Hamster protein was used as the immunogen for this Cyclin B1 antibody.

Anwendungsinformationen

Applikationshinweise: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IP: 1-2 μg/500 μg protein--precipitates active Cyclin B1 complexes

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: G2/mitotic cyclin-B1

Hintergrund:

Target Description: This antibody recognizes a protein of 55-62 kDa, identified as Cyclin B1. In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.

Gene Symbol: CCNB1///CCNB

Gen-ID: 891

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase