Topoisomerase II alpha Antikörper (AA 1352-1493)

Produktnummer ABIN6294307

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Topoisomerase II alpha in WB, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für Topoisomerase II alpha Antikörper (AA 1352-1493) (ABIN6294307)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: AA 1352-1493

Verwendungszweck: Mouse anti-Human/Mouse TOP2A Antibody / Topoisomerase II alpha [Sodium Azide Free]

Spezifität: Nuclear

Aufreinigung: Amino acids 1352-1493 from the human protein were used as the immunogen for this Topoisomerase II alpha antibody.

Immunogen: Amino acids 1352-1493 from the human protein were used as the immunogen for this Topoisomerase II alpha antibody.

Anwendungsinformationen

Applikationshinweise: FACS: 0.5-1 μg/10^6 cells in 0.1ml
IF: 1-2 μg/mL
Western blot: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: DNA topoisomerase 2-alpha

Hintergrund:

Target Description: This mAb recognizes an ~170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Gene Symbol: TOP2A///TOP2

Gen-ID: 7153

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases