TOP1MT Antikörper (Internal Region)

Produktnummer ABIN6265672

Dieses Anti-TOP1MT-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TOP1MT in WB und ELISA. Geeignet für Human, Maus und Ratte.

Kurzübersicht für TOP1MT Antikörper (Internal Region) (ABIN6265672)

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TOP1MT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TOP1MT Antikörper

Bindungsspezifität: Internal Region

Spezifität: TOP1MT Antibody detects endogenous levels of total TOP1MT.

Homologie: Pig,Zebrafish,Horse,Sheep,Chicken

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TOP1MT, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu TOP1MT

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Andere Bezeichnung: TOP1MT

Hintergrund:

Description: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

Gene: TOP1MT

Molekulargewicht: 70 kDa

Gen-ID: 116447

UniProt: Q969P6