ERCC3 Antikörper (N-Term)

Produktnummer ABIN6261590

Produktdetails anti-ERCC3 Antikörper

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Bindungsspezifität: N-Term

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERCC3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Spezifität: ERCC3 Antibody detects endogenous levels of total ERCC3.

Homologie: Pig,Zebrafish,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ERCC3, corresponding to a region within N-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu ERCC3

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Andere Bezeichnung: ERCC3 (ERCC3 Produkte)

Synonyme: BTF2 antikoerper, GTF2H antikoerper, RAD25 antikoerper, TFIIH antikoerper, XPB antikoerper, BTF2 p89 antikoerper, Ercc-3 antikoerper, wu:fc25f08 antikoerper, ERCC excision repair 3, TFIIH core complex helicase subunit antikoerper, excision repair cross-complementing rodent repair deficiency, complementation group 3 antikoerper, excision repair cross-complementation group 3 antikoerper, ERCC3 antikoerper, Ercc3 antikoerper, ercc3 antikoerper

Hintergrund:

Description: ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.

Gene: ERCC3

Molekulargewicht: 89kDa

Gen-ID: 2071

UniProt: P19447

Pathways: DNA Reparatur