ERCC3 Antikörper (AA 242-261)

Produktnummer ABIN351537

Produktdetails anti-ERCC3 Antikörper

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Bindungsspezifität: AA 242-261

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ERCC3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Recognizes an epitope located between aa242-261 of ERCC-3 (largest subunit of TFIIH).

Aufreinigung: Protein G purified

Immunogen: Synthetic peptide corresponding to aa242-261 of human ERCC-3.

Type of Immunogen: Synthetic peptide

Klon: 3G4

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (5 - 10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunoprecipitation: Immunoprecipitates transcriptionally active TFIIH in a reconstituted assay.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-HCl,  pH 7.5. Sourced from ascites.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ERCC3

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Andere Bezeichnung: ERCC3 / XPB (ERCC3 Produkte)

Synonyme: BTF2 antikoerper, GTF2H antikoerper, RAD25 antikoerper, TFIIH antikoerper, XPB antikoerper, BTF2 p89 antikoerper, Ercc-3 antikoerper, wu:fc25f08 antikoerper, ERCC excision repair 3, TFIIH core complex helicase subunit antikoerper, excision repair cross-complementing rodent repair deficiency, complementation group 3 antikoerper, excision repair cross-complementation group 3 antikoerper, ERCC3 antikoerper, Ercc3 antikoerper, ercc3 antikoerper

Hintergrund: Name/Gene ID: ERCC3

Synonyms: ERCC3, BTF2, GTF2H, RAD25, TFIIH p89, TFIIH, XPB, BTF2 p89, XPBC, TFIIH 89 kDa subunit

Gen-ID: 2071

UniProt: P19447

Pathways: DNA Reparatur