DNA2 Antikörper (Internal Region)

Produktnummer ABIN6261326

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch DNA2 in ELISA, WB, IF und ICC. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für DNA2 Antikörper (Internal Region) (ABIN6261326)

Target: DNA2 (DNA Replication Helicase 2 Homolog (DNA2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DNA2 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-DNA2 Antikörper

Bindungsspezifität: Internal Region

Spezifität: DNA2L Antibody detects endogenous levels of total DNA2L.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DNA2L, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu DNA2

Target: DNA2 (DNA Replication Helicase 2 Homolog (DNA2))

Andere Bezeichnung: DNA2

Hintergrund:

Description: Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

Gene: DNA2

Molekulargewicht: 120 kDa

Gen-ID: 1763

UniProt: P51530

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA