GFI1B Antikörper (Internal Region)

Produktnummer ABIN6258819

Der Kaninchen Polyklonal Anti-GFI1B-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, GFI1B in Proben von Human und Maus zu detektieren.

Kurzübersicht für GFI1B Antikörper (Internal Region) (ABIN6258819)

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFI1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-GFI1B Antikörper

Bindungsspezifität: Internal Region

Spezifität: GFI1B Antibody detects endogenous levels of total GFI1B.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human GFI1B, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Haltbarkeit: 12 months

Details zu GFI1B

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Andere Bezeichnung: GFI1B

Hintergrund:

Description: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context, represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1, GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription, as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.

Gene: GFI1B

Molekulargewicht: 43 kDa

Gen-ID: 8328

UniProt: Q5VTD9

Pathways: Cellular Response to Molecule of Bacterial Origin