ASK1 Antikörper (pSer966)

Produktnummer ABIN6255571

Dieses Anti-ASK1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ASK1 in WB, IHC, ELISA, IF und ICC. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für ASK1 Antikörper (pSer966) (ABIN6255571)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ASK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-ASK1 Antikörper

Bindungsspezifität: pSer966

Spezifität: Phospho-ASK1 (Ser966) Antibody detects endogenous levels of ASK1 only when phosphorylated at Serine 966.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human ASK1 around the phosphorylation site of Ser966.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Referenzen für anti-ASK1 Antikörper (ABIN6255571)

Feng, Li, Du, Sun, Feng, Li, Liu, Wang, Liu, Amizuka, Li: "Dual function of peroxiredoxin I in lipopolysaccharide-induced osteoblast apoptosis via reactive oxygen species and the apoptosis signal-regulating kinase 1 signaling pathway." in: Cell death discovery, Vol. 4, pp. 47, (2018) (PubMed).

Details zu ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Andere Bezeichnung: MAP3K5

Hintergrund:

Description: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).

Gene: MAP3K5

Molekulargewicht: 155kDa

Gen-ID: 4217

UniProt: Q99683

Pathways: MAPK Signalweg, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response