APC Antikörper (pSer2054)

Produktnummer ABIN6255083

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch APC in WB, IHC, ELISA, IF und ICC. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für APC Antikörper (pSer2054) (ABIN6255083)

Target: APC (Adenomatous Polyposis Coli (APC))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-APC Antikörper

Bindungsspezifität: pSer2054

Spezifität: Phospho-APC (Ser2054) Antibody detects endogenous levels of APC only when phosphorylated at Sersine 2054.

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human APC around the phosphorylation site of Ser2054.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu APC

Target: APC (Adenomatous Polyposis Coli (APC))

Andere Bezeichnung: APC

Hintergrund:

Description: Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Gene: APC

Molekulargewicht: 311kDa

Gen-ID: 324

UniProt: P25054

Pathways: WNT Signalweg, Stem Cell Maintenance, Cell-Cell Junction Organization