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APEX1 Antikörper

Dieses Anti-APEX1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von APEX1 in WB. Geeignet für Arabidopsis thaliana und Spinach. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.
Produktnummer ABIN6254847

Kurzübersicht für APEX1 Antikörper (ABIN6254847)

Target

Alle APEX1 Antikörper anzeigen
APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reaktivität

  • 97
  • 48
  • 44
  • 8
  • 7
  • 7
  • 7
  • 6
  • 6
  • 6
  • 5
  • 4
  • 2
  • 1
  • 1
Arabidopsis thaliana, Spinach

Wirt

  • 80
  • 19
  • 2
Kaninchen

Klonalität

  • 80
  • 21
Polyklonal

Konjugat

  • 71
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser APEX1 Antikörper ist unkonjugiert

Applikation

  • 88
  • 34
  • 31
  • 27
  • 22
  • 16
  • 8
  • 8
  • 8
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Produktmerkmale

    Expected / apparent Molecular Weight of the Antigene: 25-38 kDa for A. thaliana
    Antibody detection limit is 7 pg, 20 fmol, linear range of logit/logplot is 0.023-15pmol,midrange (B/Bo=50%) is 120 pg, 342 fmol .

    Aufreinigung

    serum

    Immunogen

    synthetic peptide derived from Arabidopsis thaliana tAPX (acession At1g77490) and sAPX (At1g08390) protein sequences, coupled to BSA. Five out of twelve amino acids are also identical with cAPX1 (At1g07890), cPX2 (At1g09640) and pAPX (At1g35000)
  • Applikationshinweise

    Recommended Dilution: 1 : 2000 with standard ECL (WB).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    For reconstitution add 200 µL of sterile water

    Handhabung

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    Lagerung

    -20 °C
  • Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

    Lehtima: "Nodularin uptake and induction of oxidative stress in spinach (Spinachia oleracea)." in: Journal of plant physiology, (2010) (PubMed).

    Hideg, Kuos, Schreiber: "Imaging of NPQ and ROS formation in tobacco leaves: heat inactivation of the water-water cycle prevents down-regulation of PSII." in: Plant & cell physiology, Vol. 49, Issue 12, pp. 1879-86, (2008) (PubMed).

    Kangasjaervi, Lepistoe, Haennikaeinen, Piippo, Luomala, Aro, Rintamaeki: "Diverse roles for chloroplast stromal and thylakoid-bound ascorbate peroxidases in plant stress responses." in: The Biochemical journal, Vol. 412, Issue 2, pp. 275-85, (2008) (PubMed).

  • Target

    APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

    Andere Bezeichnung

    APX

    Hintergrund

    AGI Code: At1g77490
    APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water. Cellular localization includes chloroplast (tAPX and sAPX), cytosol (cAPX) and peroxisome (pAPX).

    Molekulargewicht

    25-38 kDa for A. thaliana

    UniProt

    Q42564, Q05431, Q1PER6, Q42592, Q42593

    Pathways

    DNA Reparatur, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
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