Neurofilament Antikörper (Extracellular Domain)

Produktnummer ABIN6252792

Dieser Maus Monoklonal Antikörper detektiert spezifisch Neurofilament in IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Neurofilament Antikörper (Extracellular Domain) (ABIN6252792)

Target: Neurofilament

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Neurofilament Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: SPM563

Produktdetails anti-Neurofilament Antikörper

Bindungsspezifität: Extracellular Domain

Aufreinigung: Protein G affinity chromatography

Immunogen: A membrane preparation of small cell lung carcinoma was used as the immunogen for this anti-CD56 antibody. This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the anti-CD56 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Neurofilament

Target: Neurofilament

Hintergrund: Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135  kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Gen-ID: 4684

UniProt: P13591