Neurofilament Antikörper

Produktnummer ABIN6252790

Der Maus Monoklonal anti-Neurofilament Antikörper wird verwendet zum Nachweis von Neurofilament in Proben von Human, Ratte, Maus, Huhn und Schwein. Er wurde validiert für FACS, IHC und WB.

Kurzübersicht für Neurofilament Antikörper (ABIN6252790)

Target: Neurofilament

Reaktivität: Human, Ratte, Maus, Huhn, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Neurofilament Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)

Klon: NF421

Produktdetails anti-Neurofilament Antikörper

Aufreinigung: Protein G purified Neurofilament antibody

Immunogen: Human recombinant Neurofilament protein was used as the immunogen. (1)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (2)

Prediluted format : incubate for 30 min at RT (3)

The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.

1. The NF421 antibody is specific to the heavy subunit.
2. FFPE tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Neurofilament

Target: Neurofilament

Hintergrund: Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68  kDa (NF-L), 160  kDa (NF-M) and 200  kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.

Gen-ID: 4744

UniProt: P12036