TOP2A
Reaktivität: Human
WB, ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml
IF: 1-2 μg/mL
Western blot: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Target
Topoisomerase II alpha (TOP2A)
(Topoisomerase (DNA) II alpha 170kDa (TOP2A))
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Hintergrund
This mAb recognizes an ~170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.