Topoisomerase II alpha Antikörper (AA 1352-1493)

Produktnummer ABIN6252772

Der Maus-Monoklonal Anti-Topoisomerase II alpha-Antikörper wurde für WB, IHC, IF und FACS validiert. Er ist geeignet zum Nachweis von Topoisomerase II alpha in Proben aus Human und Maus. }

Kurzübersicht für Topoisomerase II alpha Antikörper (AA 1352-1493) (ABIN6252772)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: TOP2A-1361

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: AA 1352-1493

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids 1352-1493 from the human protein were used as the immunogen for this Topoisomerase II alpha antibody.

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml

IF: 1-2 μg/mL

Western blot: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A

Hintergrund: This mAb recognizes an ~170  kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Gen-ID: 7153

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases