Keratin Basic Antikörper

Produktnummer ABIN6252470

Dieses Anti--Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von in WB, IF, FACS, IHC (p) und ELISA. Geeignet für Human und Ratte.

Kurzübersicht für Keratin Basic Antikörper (ABIN6252470)

Target: Keratin Basic

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA

Klon: KRTH-1076

Produktdetails

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human KRT76 protein was used as the immunogen for the Basic Cytokeratin antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: ELISA (For coating order Ab without BSA)Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Western blot: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the Basic Cytokeratin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Antigendetails

Target: Keratin Basic

Andere Bezeichnung: Basic Cytokeratin

Hintergrund: This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67  kDa (CK1), 64  kDa (CK3), 59  kDa (CK4), 58  kDa (CK5), 56  kDa (CK6), 52  kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40  kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Gen-ID: 51350

UniProt: Q01546