Keratin Basic Antikörper

Produktnummer ABIN3023995

Der Maus Monoklonal anti-Keratin Basic Antikörper (Klon AE3) (ABIN3023995) detektiert spezifisch Keratin Basic in WB, IHC (p), IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für Keratin Basic Antikörper (ABIN3023995)

Target: Keratin Basic

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Carrier-free

Klon: AE3

Produktdetails

Verwendungszweck: Basic Cytokeratin Antibody (azide and preservative free)

Aufreinigung: Protein G purified

Immunogen: Solubilized cytokeratin extract from human stratum corneum was used as the immunogen.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Differences in protocols and secondaries may cause the Basic Cytokeratin antibody to need titering for optimal performance.

1. FFPE staining requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Antigendetails

Target: Keratin Basic

Andere Bezeichnung: Basic Cytokeratin

Hintergrund: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The basic family clone AE3 is commonly used in combination with clone AE1 as a pan cytokeratin antibody acidic + basic cocktail (< a href=../pan-cytokeratin-antibody-cocktail-ae1-ae3-v2330>Cat No V2330).