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Rekombinanter Keratin Basic Antikörper

Reaktivität: Human WB, IF, FACS, IHC (p) Wirt: Kaninchen Monoclonal KRTH-1576R unconjugated Recombinant Antibody
Produktnummer ABIN4949251
  • Target Alle Keratin Basic Antikörper anzeigen
    Keratin Basic
    Antikörpertyp
    Recombinant Antibody
    Reaktivität
    • 21
    • 13
    • 10
    • 8
    • 8
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 14
    • 8
    Kaninchen
    Klonalität
    • 22
    Monoklonal
    Konjugat
    • 14
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Unkonjugiert
    Applikation
    • 19
    • 17
    • 15
    • 14
    • 9
    • 5
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Aufreinigung
    Protein A affinity chromatography
    Immunogen
    Recombinant human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody.
    Klon
    KRTH-1576R
    Isotyp
    IgG kappa
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  • Applikationshinweise
    Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Konservierungsmittel
    Azide free
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store the recombinant Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Keratin Basic
    Andere Bezeichnung
    Basic Cytokeratin (Keratin Basic Produkte)
    Hintergrund
    This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67  kDa (CK1), 64  kDa (CK3), 59  kDa (CK4), 58  kDa (CK5), 56  kDa (CK6), 52  kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40  kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
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